19^th World Congress on Biotechnology November 13-14, 2017 Osaka, Japan

Biography:

Dr. Mehmet Cengiz Baloglu is Associate Professor of Department of Genetic and Bioengineering at Kastamonu University. He received his PhD in 2011 at Middle East Technical University (METU). His PhD thesis was awarded as the best thesis in METU. He has established molecular biology, transcriptomics and bioinformatics research laboratories at the Kastamonu University in 2013. Dr. Baloglu has published over 50 peer-reviewed manuscripts on topics concerning with gene identification in plants, transcriptome and miRNA analysis using NGS and abiotic stress in plants. He is serving as an Associate editor and Editorial Board member for international journals with high impact factors.

Abstract:

Triticum monococcum is the domesticated form of einkorn wheat which is a staple food of early farmers for many thousand years. Siyez is one of the most famous einkorn wheat cultivar and domesticated in Kastamonu, Turkey. MicroRNAs (miRNAs) are known as short non-coding RNAs that regulate gene expression at post-transcriptional level. In this study, miRNAs and their target genes were determined after drought stress in leaf and root samples of Siyez. Four small RNA libraries were constructed. Sequencing was performed on the Illumina HiSeq 2500. All bioinformatics analysis was carried out using CLC Genomics Workbench v.10. Although there are many studies related with transcriptome analyses in wheat, microRNA deep sequencing under drought stress in einkorn wheat has been firstly performed. After drought treatment, expression level of 30 and 69 miRNAs were increased and decreased, respectively. Expression level of tae-miR9672a-3p, tae-miR9666a-3p, tae-miR9773, tae-miR5048-5p and tae-miR167c-5p were significantly raised, whereas tae-miR395a/b, tae-miR9674a-5p, tae-miR9662b-3p, tae-miR159a/b and tae-miR5200 expressions were remarkably reduced after drought stress application in Siyez. To identify potential regulatory effects of miRNAs on mRNAs, target genes were also predicted for all libraries. A detailed bioinformatics analysis showed that miRNAs upregulated in response to drought stress, such as tae-miR9661-5p, tae-miR156 primarily targeted transcripts associated with activation of immune regulator SRFR1 and SBP-box genes. In the current study, a temporal miRNA-guided post-transcriptional regulation responded to drought was observed in leaf and root tissues of einkorn wheat. This kind of stress induced miRNA identification studies open new perspectives for designing novel wheat breeding strategies.

Biography:

Pınar Baloglu is a biologist at Kastamonu University in Research and Application Center. She received her M.Sc. in 2016 at Department of Genetic and Bioengineering in Kastamonu University. In her thesis, she has identified 79 LEA (Late Embryogenesis Abundant) genes in cucumber genome. It was also tested their response against to drought stress in cucumber. Expert Biologist Baloglu has published 2 manuscripts on topics concerning with gene familes identification in different plants. She has also attended many congress related with molecular biology and bioinformatics. Now, she is a Ph.D. candidate in same department.

Abstract:

Wheat is an annual herbaceous plant whose breeding is done all over the world. Siyez wheat (Tiriticum monococcum) with 2n chromosomes is the first wheat variety which is known the oldest and taken culture. bZIP transcription factors have DNA binding motifs and form the leucine zipper dimerization. Most of WRKY type transcription factors control regulation of important functions for the development of plants. In this study, transcription factor genes were firstly cloned form einkorn wheat. For this, genes were amplified in PCR using gene specific primers with Pfu Taq Polymerase enzyme. PCR products were transferred to pENTR™/D-TOPO^® input vector. Plasmids containing genes were sent to sequencing. Finally, the sequences of the genes were aligned using CLC Genomics Workbench bioinformatics program. bZIP gene with 450 bp in length and WRKY gene with 672 bp in length were successfully cloned in a Gateway-compatible input vector. DNA sequence of genes were translated to protein sequence. A total of 150 amino acids in length for bZIP proteins and a total of 224 amino acids in length for WRKY proteins were determined. 3-D structure of proteins were modeled using Phyre2 program. The structural differences of these proteins in Siyez were identified. With this study, the sequence of bZIP and WRKY type transcription factors genes were firstly determined in ancestral wheat cultivar, Siyez and tried to obtain information about the function of these genes. Obtained results from this study may be used for development of abiotic stress resistance plants.

Biography:

Dr. Mehmet Cengiz Baloglu is Associate Professor of Department of Genetic and Bioengineering at Kastamonu University. He received his PhD in 2011 at Middle East Technical University (METU). His PhD thesis was awarded as the best thesis in METU. He has established molecular biology, transcriptomics and bioinformatics research laboratories at the Kastamonu University in 2013. Dr. Baloglu has published over 50 peer-reviewed manuscripts on topics concerning with gene identification in plants, transcriptome and miRNA analysis using NGS and abiotic stress in plants. He is serving as an Associate editor and Editorial Board member for international journals with high impact factors.

Abstract:

Advent of next generation sequencing (NGS) has been dramatically altered the fields of omics technologies including genomics, transcriptomics, proteomics and metabolomics. NGS provides mass analysis for genome and transcriptome of organisms including plants. This advanced technique accelerates identification of genes, regulatory sequences and biomarkers found in plant genomes. Although many draft plant genome sequences have been published over the past decade, all genes in genomes have not been completely detected. Therefore, gene family identification studies have been still continued. Gene families are often spread in the genome through tandem and segmental arrangements. Orthologous-paralogous genes, transcription factor family genes, some simple sequence repeats, microsatellites, microRNAs and long intergenic noncoding RNAs are in great demand for genome survey researchers. Development of new bioinformatics tools have caused detection of these important sequences in plant genomes. Transcriptome, molecular breeding, genetic transformation and genome editing studies can be considered as samples for this application in plant biotechnology. RNA-sequencing technology has been widely used for both measurement of gene expression levels and discovery of new genes and new alternative splicing isoforms. Aim of modern plant breeding programs is to improve and increase some agronomically important traits. The most significant contribution of genome wide investigation studies has provided precious source for functional genomics and plant breeding programs. Genome editing strategies including genetic transformation, RNA interference and CRISPR/Cas9 have a great potential to obtain new plant phenotypes with desirable characteristics. In the future, new omics analysis tools will be emerged and will provide deep investigation for plant genomes.

Biography:

Dinh Thi My Huong completed her Barchelor from Da Nang University of Technology, recently she studies master program of Biochemistry at from Ming Chi Unviersity of Taiwan. She is the lecture of Da Nang collegue of Technology.

Abstract:

Water is the factor need to attend on the esterification to produce biodiesel by enzyme catalysts because of its complicated influences on the catalyst activity and sability of enzyme as well as the productivity of reaction. Some studies conclued that some lipases need to be maintained its proper structure by the certain amount of water. On the other hand, water is the by-product of the ester reaction, hence the high water content exists in the reaction media causes the back forward reaction, consequeces to reduce the yield. Therefore, this study drive to indentify the suitable amount of water for assisting activition of liquid enzyme catalyst, and investigate the using SAP to absorb the produced water. The effect of water content on the enzyme activity was assigned through discussed the efficiency of the esterifiaction with molar ratio of oleic acid to methanol 1:3 at the temperature of 250C, catalyst amount of 10wt% and varied water amount of 0 to 30 wt% (base on the fatty acid mass) for 1 hour and 3 hours. Another rections were carried out with the condition similar to above reactions and added 5% (w/woleic acid) of SAP amount to study the ability of maintaining the esterifiaction. Results illustrated that the conversion was increased obivously from 0% to 95 % with added water content increased from 0 wt% to 30wt%. Meanwhile the conversion rate was improved significant with using SAP, the yield reached to 95% with added water ammount of 10 wt% for only 1 hour.
 

Biography:

Dr. A. Vieira completed his BSc and PhD studies in Alberta, Canada, and postdoctoral studies in California, USA. He is currently Associate Professor, and Director of the Nutrition and Metabolism Research Laboratory, Biomedical Physiology (BPK) Department, Simon Fraser University, Burnaby, Canada. He has over 90 publications, including research papers in major international journals, with over 1500 citations. Dr. Vieira has served as reviewer and editorial board member for journals related to biomedical research, molecular and cellular biology, as well as educational and scientific books.

Abstract:

Modulators of cell functions such as transport pathways, signal transduction, and redox balance may have biomedical applications as pharmaco-therapeutics. We have developed biochemical and cellular high-throughput screening assays to assess activities of natural products, standardized plant extracts, as well as synthetic compounds and biosimilars. Several of these assays will be discussed in the context of (a) pro-oxidative and other pathological effects of misfolded and aggregated polypeptides in amyloidogenic diseases, (b) infectious diseases involving endocytosis of the microbe, and (c) epigenetic regulation in metabolic disorders. Medicinal and dietary plant extracts, purified phytochemicals including flavonoids and nutrients, as well as combinations of purified compounds, are currently undergoing screening in our laboratory; and the latest results will be presented.

Biography:

Professor Kim and Kyu-yeon Lee have been studying on the neutralization ability of acid soil and the environmental impacts and decomposition mechanisms of micro pollutants such as medicines and micro-plastics in Soil Groundwater Laboratory of Inha University. Also, investigating on the characteristics of microbial diversity in natural radon soil environments verifying microbial differences at various radon concentration conditions.

Abstract:

Radon is an inert gas with no color and odor having a half-life of 4 days, which is a radioactive element produced by the decay of Uranium. Recently, public interest about indoor radon presence has been increased over decade. In general, high concentrations radon is generally known to be making deleterious effects on plants, animals and humans, which can cause cell viability disruption, cell morphological changes or hormonal disorders. On the contrary, lower concentration of radon may nevertheless improve crop growth while disabling pest activity. This study shows how much of lower level concentrations of radon in natural soils affect microbial community and their diversity with regard to basal soil physicochemical characteristics. Microorganisms exposed to low radioactivity, such as low-level radon, can have strong viability and high biodiversity. Soil physicochemical parameters such as pH, electrical conductivity, moisture content and soil particle size were measured according to Korean Standard Analytical Methods for Soils. Gas phase of radon concentration was measured for 1 hour (FRD-400, FT-Radon Lab., Korea) while the concentration of it has been varied in lower, equal and greater level compared to the natural source of radon origin in the field. In the meantime, colony enumeration, dehydrogenase activity, and identification of species were performed. In the lon run, there were relatively greater extent of diversity and population density being observed when microbes were exposed to relatively lower or equal level compared to the natural origin. In response, they revealed higher enzymatic activity under the given lower level radon exposure.

Biography:

Supamas Napavichayanun is a PhD student, faculty of Pharmaceutical Sciences, Chulalongkorn University, Thailand. She earned a B.Sc. from faculty of Phamaceutical sciences, Chulalongkorn University in 2010. Her research experience has ranged from protein including silk proteins and biomaterials. She also did clinical researches in the area of dermatology especially materials for wound healing application.

Abstract:

Wound dressing is an important factor for wound treatment. The good properties of wound dressing will lead to accelerate wound healing. Therefore, the objective of this study was to develop the bacterial cellulose wound dressing containing silk sericin and polyhexamethylene biguanide (PHMB)  for wound treatment. Coconut is one of the value product of Thailand’s industrial harvests. Unfortunately, ripe coconut water is usually a waste product from coconut milk production  that is discarded into the environment and results in pollution. Bacterial cellulose is produced by Acetobacter xylinum which is fermented in ripe coconut water. It has many advantages for wound healing: transparency, autolytic debridement, acceleration of re-epithelialization, and fewer daily wound dressing changes. Because of the many benefits of bacterial cellulose, it is used in many applications including cosmetics and medical devices such as wound treatment dressing material. Silk sericin is a protein from silk cocoons which can accelerate the proliferation of fibroblast cells and activate collagen synthesis for wound healing. PHMB is a broad-spectrum antimicrobial agent with high efficacy and  low toxicity. Accordingly, the combination of bacterial cellulose wound dressing containing silk sericin and PHMB will have many benefits to the wound.  The safety and efficacy of the dressings, in vitro and in vivo was investigated. The results showed the dressing has good physical, mechanical, and biological properties. The wound size of wounds treated with the dressing showed a significantly less than control. No inflammation or irritation was shown in rats. Moreover, in the clinical study, the dressing also showed many benefits for split–thickness skin graft wound treatment without any toxicity.

Biography:

Gladys Hayashida has completed recently her PhD from Antofagasta University and her Master studies from Kyoto University. She is the director of the Associative Regional Project Explora of CONICYT, a Science Promotion Grant supported by the Goberment of Chile and implemented by the Antofagasta University. She has published scientific articles related to bioactive substances from marine bacteria and microalgal biotechnological applications, in reputed journals and has been her participating as researcher in several scientific studies.

Abstract:

Microalgae are an important source of unsaturated fatty acids, phospholipids, glycolipids, and carotenes, which are useful compounds for the food and pharmaceutical industries. The Atacama Desert of northern Chile is one of the driest deserts on Earth and, as such, it is a great natural laboratory in which to study new microorganisms adapted to extreme environments. A microalgal strain, referred to here as CH03, was isolated from a microbial mat in salt flat water in Salar de Atacama. Genetic analysis of the 18S ribosomal RNA gene showed that the strain had homology with other known sequences of the species Chlorella sorokiniana. Results revealed the adaptability of this microalga to freshwater medium under laboratory conditions, despite coming from an extremely high-salinity environment. The fatty acid profile of CH03(A) newly isolated in Bold's basal medium differed from that of CH03(B) cultured in vitro in modified F/2 medium and from another five strains of C. sorokiniana and three strains of Chlorella vulgaris in that it had a high stearic acid content and had no polyunsaturated fatty acids. The major biochemical components observed in this strain were proteins (64.3–73.6%) and lipids (26.6–32.6%). This study suggests that the strain CH03 could be a protein source and that this oleaginous microalga is easy to grow in vitro as a biological model for future studies.

Biography:

Dr. Chien-Hsun Huang is now an associate researcher in Fudan University, China. He received his Ph.D. degree of Plant Biology in the Institute of Plant Biology, Department of Life Sciences, National Taiwan University, and started to study plant evolution in bioinformatics in his postdoc position. He accumulated his experiences in molecular biology, biochemistry and bioinformatics. He has involved in several government-granted projects of NSFC.
 

Abstract:

Bioinformatics is getting vital in the genomic era with the advance of sequencing tecniques leading to large amounts of datasets generated around the world. Similarly is the emerging importance of the comparative studies, instead of focusing on understanding one particular model species in previous days, to find the mechanisms basal and universal to most of the species as well as the ones specified to a few organisms. However, a well-resolved and robust phylogeny is required for many of the comparative studies to make assumptions as well as possible. Thus, we focus on resolving the phylogeny of angiosperms, hoping to provide a framework for comparative studies in plants. Using large numbers of transcriptomic datasets, we selected hundreds of low-copy or single-copy nuclear genes to reconstruct the phylogeny of several angiosperm families such as Asteraceae, Rosaceae and Brassicaceae. In these studies, we first provide a solid relationships among members of these families by using different approaches to confirm the results, and then reconstruct the history of morphological characters or gene/genome duplications based on the model phylogeny. We have found repeated and nested whole-genome duplications in Asteraceae, and also revreal the evolutionary history of fruit type in Rosaceae. Furthermore, we especially focus on and anticipate to provide a solid framework and sufficient information for Brassicaceae to be used as a “model family” in plant biology.

Biography:

Khosrow Aghaiypour Kolyani has completed his PhD at the age of 30 years from Tehran University of Medical Sciences and postdoctoral studies from national Institute of Health, National Cancer Institute at frederick, USA. He is the head of Genomics and Genetic Engineering Department of Razi Vaccine and Serum Research Institute, which organizes the main human and animal vaccine research in Iran. He has published more than 30  papers in reputed journals and has been serving as an editorial board member of repute.

Abstract:

Hyalomma anatolicum anatolicum (H. a. anatolicum) as the most widespread tick species in Iran and other parts of the middle east is responsible for the hugely serious economic losses in livestock industry. This study was conducted to investigate genetic variability of the Bm86 orthologous gene, HA03, in five different Iranian H. a. anatolicum isolates including Kordan, Qom, Boinzahra, Lorestan and Bushehr. Likewise, a number of in silico analyses were performed in order to predict the possible impact of the amino acid substitutions on antigenicity of the protein. Comparative sequence analysis of the Bm86 orthologous gene sequence among five tick isolates allowed for identification of four non-synonymous single nucleotide polymorphisms (SNPs) including c.995A > C, c.1150G > C, c.1151A > C/T and c.1152G > T which would result in p.Asn 332 Thr, p.Glu 384 Leu and p.Glu 384 Ala substitutions. As much as antigenicity is concerned, based on our in silico studies, the amino acid position 384 was located in a putative antigenic peptide of the protein. Our subsequent physicochemical and structural analyses illustrated that two out of three amino acid substitutions including p.Glu 384 Leu and p.Glu 384 Ala considerably influenced the 3-dimensional structure and physicochemical properties of HA03 protein including hydrophobicity, amphiphilicity and net charge; thus, they might affect the antigen-antibody reaction and consequently immunogenicity of the antigen. In conclusion, it is a rational measure not only to replace Bm86 with HA03 in formulation of the recombinant anti-tick vaccine, but also to combine various antigens extracted from different isolates of the tick species.

Biography:

Leslie Retnam completed his B. Vet. Science from the University of Queensland, Australia and Masters in Laboratory Animal Science from Hahnemann University, Philadelphia, USA. He is Director of Veterinary Services at Biological Resource Centre (BRC), Agency for Science, Research and Technology (A*STAR), Singapore. BRC is located at Biopolis, which is the national research and development hub for biomedical sciences with 9 Research Institutes. He is also Council Member with the AAA

Abstract:

The use of animals in any field – be it food, work, entertainment or scientific purposes – continues to attract the attention of individuals with interest in their welfare. Of relevance to the biotechnology industry is the use of live animals for testing, research and training because of the potential harm and distress which may compromise their wellbeing. In Singapore, national guidelines regulating the use of animals for scientific purposes are currently being revised to further enhance animal welfare and promote good science.

The National Advisory Committee on Laboratory Animal Research (NACLAR) was established in 2003 to formulate a set of national policies and standards regulating the acquisition, housing and utilisation of laboratory animals in biomedical research. It also sought to address related scientific, ethical and legal issues pertaining to the use of animals within the biotechnology industry. Shortly after the publication of NACLAR’s guidelines, the Agri-Food and Veterinary Authority (AVA) announced ‘The Animals and Birds (Care for the use of animals for Scientific Purposes) Rules’ which legislated compulsary licensing of animal research facilities. To this day, these measures maintain a high standard of animal welfare within Singapore’s biotechnology industry.

This poster depicts a historical perspective on the development of guidelines and regulations in Singapore relating to the use of animals for scientific purposes, and illustrates enhancements to the first edition of the NACLAR Guidelines currently being deliberated to improve animal welfare.

Biography:

Mahasin wadi PhD Medical Microbiology, is currently working at College of Nursing , Princess Nourah Bint Abdulrahaman  University, Riyadh, Saudi Arabia. She published a numbers of papers in reputed journals and Participated in many Internationals and national conferences. She issued a patent research about the antimicrobial activity of Sudanese bee honey.  She attended many workshops and seminars. Awarded many appreciation and thanks certificate in scientific activities.  Member of many international  associations : German Apitherapy Society, American Apitherapy Society, International Bee Research Association , European Society of Clinical Microbiology and Infectious Disease ESCMID . She  served as reviewer of various journals. Her research interest area is the antibacterial and haling effects of Bee honey as natural product.

Abstract:

Background: The therapeutic value of honey was underlined in various literature. Honey was widely used in folk medicine throughout the world. Honey has been used to treat a number of clinical conditions: treatment of burns, wounds, peptic ulcers, gastritis, eye infection and sore throat. Antibacterial  activity  is  attributed  partially to  the  high osmolality of  the  sugar  content  of  honey  (Crane , 1975).

Additional antibacterial  activity  of  honey was  investigated  by  extraction  and  fractionation of  honey by  organic   solvents. Honey; ethyl  acetate extract revealed potent  antibacterial  activity (Wadi, 2016).

Objectives: The objective of the current prospective study is to determine the antimicrobial activity of fifteen different honey samples collected from different floral origin. To verify the nature of active fraction of bee honey.

Methods: Fifteen different raw bee honey samples were obtained from different countries, as well as commercially sold honey samples from the local market different brands, of different floral origin. In vitro antibacterial activity of bee honey, petroleum ether , diethyl ether and ethyl acetate extracts of honey samples  were  tested against  five standard organisms ; Staphylococcus aureus : ATCC 29213, Staphylococcus Methicillin Resistant (MRSA), ATCC: 23591 Escherichia coli  :ATCC 25922 Klebsiella pneumoniae : ATCC 700603 and Pseudomonas aeruginosa ATCC 27853 .

Results: All honey samples exerted inhibitory effects on both Gram –positive and negative organisms. The petroleum ether and diethyl ether fraction exerted no activity, while the aqueous residue exerted strong antibacterial activity. Ethyl acetate fraction showed strong antibacterial activity.

Conclusion:  The  findings of the current study confirmed  that all honey samples exerted strong antibacterial activity. The chemical findings of this  study indicates the presences of polar antibacterial agent (s) which is characterized by its extractability by ethyl acetate to the organic phase (Wadi 2016). 

Biography:

Saeed Kaboli has completed his PhD and postdoctoral degrees in Department of Biotechnology, Osaka University-Japan. In molecular biology and biotechnology lab, he engaged in a project entitled “Development of novel genome engineering technology and its application in bioscience and biotechnology”. Specifically, he involved in CRISPR/Cas9 system to disclose genome function. Breeding of strains improved in industrially and medically important phenotypes also was comprised in his research. Currently, he is a Postdoctoral Researcher in Sciences and Biological Technologies, Shahid Beheshti University-Iran.
 

Abstract:

PCR-mediated chromosome splitting (PCS) was developed in the yeast Saccharomyces cerevisiae. It is based on homologous recombination and enables division of a chromosome at any point to form two derived and functional chromosomes. However, because of low homologous recombination activity, PCS is limited to a single site at a time, which makes the splitting of multiple loci laborious and time-consuming. Here we have developed a highly efficient and versatile chromosome engineering technology named CRISPR-PCS that integrates PCS with the novel genome editing CRISPR/Cas9 system. This integration allows PCS to utilize induced double strand breaks to activate homologous recombination. CRISPR-PCS enhances the efficiency of chromosome splitting approximately 200-fold and enables generation of simultaneous multiple chromosome splits. We propose that CRISPR-PCS will be a powerful tool for breeding novel yeast strains with desirable traits for specific industrial applications and for investigating genome function.

Biography:

Xiaobei Zhan completed his PhD from Kansans State University in 2004, and is a professor of fermentation engineering from Jiangnan University.  He is the director of Industrial Microbiology and Biological Reaction Engineering Research Center, School of Biotechnology, Jiangnan University and the deputy Director of Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education. He is also the ASAE and IFT Institute of United States. His research focus is technology of high-viscosity fermentation, biosynthesis of microbial polysaccharide, preparation of functional oligosaccharide and design of bioreactor. He has published more than 30 papers in reputed journals.

Abstract:

The serious envelopment of curdlan on Agrobacterium sp. ATCC 31749 during fermentation is a major obstacle to increase curdlan production, and the compact macrostructure of curdlan produced from Agrobacterium sp. ATCC 31749 limits its application in preparing curdlan β-glucooligosaccharides. In this study, the effects of different surfactants on curdlan production by Agrobacterium sp. ATCC 31749 was investigated and several new perspectives concerned with the effect of Tween 80 promoting curdlan production and altering curdlan structure were presented. Maximum curdlan production (51.94 g/L) was achieved when 16 g/L Tween-80 was added at the beginning of the cell growth stage. The addition of Tween-80 at higher concentration inhibited cell growth. However, the addition of 16 g/L Tween-80 enhanced the production of curdlan with a looser ultrastructure, significantly weakened the envelopment of curdlan on Agrobacterium sp. ATCC 31749, altered the fine structure of cell membrane, and increased the cell membrane permeability. Compared with commercial curdlan, the curdlan with a looser ultrastructure exhibited higher substrate-binding affinity and maximum reaction rate when it was used as the substrate for preparing curdlan β-glucooligosaccharides. These findings demonstrate the mechanisms by which Tween-80 enhances curdlan production and provide a cheap and feasible approach to weaken the envelopment of water-insoluble polysaccharides on bacteria. Meanwhile, this paper provided an effective method to produce an ideal substrate so as to prepare oligosaccharides using enzymic degradation.

Biography:

Xiaobei Zhan completed his PhD from Kansans State University in 2004, and is a professor of fermentation engineering from Jiangnan University.  He is the director of Industrial Microbiology and Biological Reaction Engineering Research Center, School of Biotechnology, Jiangnan University and the deputy Director of Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education. He is also the ASAE and IFT Institute of United States. His research focus is technology of high-viscosity fermentation, biosynthesis of microbial polysaccharide, preparation of functional oligosaccharide and design of bioreactor. He has published more than 30 papers in reputed journals.

Abstract:

The serious envelopment of curdlan on Agrobacterium sp. ATCC 31749 during fermentation is a major obstacle to increase curdlan production, and the compact macrostructure of curdlan produced from Agrobacterium sp. ATCC 31749 limits its application in preparing curdlan β-glucooligosaccharides. In this study, the effects of different surfactants on curdlan production by Agrobacterium sp. ATCC 31749 was investigated and several new perspectives concerned with the effect of Tween 80 promoting curdlan production and altering curdlan structure were presented. Maximum curdlan production (51.94 g/L) was achieved when 16 g/L Tween-80 was added at the beginning of the cell growth stage. The addition of Tween-80 at higher concentration inhibited cell growth. However, the addition of 16 g/L Tween-80 enhanced the production of curdlan with a looser ultrastructure, significantly weakened the envelopment of curdlan on Agrobacterium sp. ATCC 31749, altered the fine structure of cell membrane, and increased the cell membrane permeability. Compared with commercial curdlan, the curdlan with a looser ultrastructure exhibited higher substrate-binding affinity and maximum reaction rate when it was used as the substrate for preparing curdlan β-glucooligosaccharides. These findings demonstrate the mechanisms by which Tween-80 enhances curdlan production and provide a cheap and feasible approach to weaken the envelopment of water-insoluble polysaccharides on bacteria. Meanwhile, this paper provided an effective method to produce an ideal substrate so as to prepare oligosaccharides using enzymic degradation.

Biography:

Nur Kholilatul Izzah has completed her PhD at the age of 37 years from Seoul National University (SNU) in 2014. She is researcher at Plant Breeding division, Indonesian Industrial and Beverage Crops Research Institute (IIBCRI), Indonesian Agency for Agricultural Research and Development (IAARD) under Ministry of Agriculture. In 2016, she conducted postdoctoral study at Functional Genomics Laboratory, Dept. of Plant Sciences, College of Agriculture and Life Sciences, Seoul National University for 7 months. She has published more than 8 papers in reputed journals and has been serving as an editorial team member of Journal of Industrial and Beverage Crops.

Abstract:

Cacao (Theobroma cacao L.) is known as main material for chocolate industry worldwide. Indonesia is recognized as the third largest cacao producer in the world with the total production in 2014 reached 709.331 tonnes. In order to understand cacao genomic, we conducted chloroplast genome sequencing generated by an Illumina Miseq platform. Chloroplast plays a crucial role in sustaining life on earth. The availability of chloroplast sequences could enhanced our understanding of chloroplast biology, conservation, diversity, and the genetic basis by which chloroplast transgenes can be engineered to enhance plant agronomic traits. The size of chloroplast genomes of cacao ranged from 160,619 bp to 160,649 bp. Cacao chloroplast sequences encoded 114 genes, consisted of 80 protein coding genes, 30 tRNA genes, and four rRNAs genes. Based on chloroplast sequences, we conducted phylogenetic analysis of 12 cacao genotypes that successfully separated bulk and fine types. The dendrogram resulted in this study proved the utility of chloroplast sequences for phylogenetic analysis. Some variations demonstrated through the number and structure of repetitive sequence in cacao chloroplast sequences. Identification of repetitive sequence by REPuter program exhibited that cacao possessed 18 repeats and three repeat structures (forward, palindrome, and reverse). In addition, we have developed three indel-based barcode markers which were designed based on the polymorphic regions of trnK-UUU - rps16, rps16 intron, and trnA-UGC - rrn23. The result obtained herein would give new insight regarding chloroplast genome structure in cacao, which would be useful to resolve phylogenetic relationships and development of DNA barcode markers.

Biography:

Yibing Hu completed his Ph.D.  in 2007,  from Institute of Botany, Chinese Academy of Sciences. He is an associate professor of Nanjing Agricultural University with more than 20 papers in related journals .
 

Abstract:

Using β-glucoronidase (GUS) and Green Fluorescent Protein (GFP) represented expression, CRISPR-associated gene editing, cross-fertilization and determination of sugar related physiological parameters in gene mutant lines and wild type plants, we aimed to investigate the function of sugar transporter OsSWEETs and OsSUTs in rice caryopsis development during grain filling. Currently, we demonstrated that OsSWEET11 play an essential role in sucrose release from maternal tissue to the maternal–filial interface. It might also induce sucrose release from the ovular vascular trace and cross cells of developing caryopsis. In addition, OsSWEET15, a homolog of OsSWEET11 in rice SWEET family, also play an important part in grain filling besides its prominent role in pollen development of rice. By contrast, the sucrose-proton symporters OsSUT1/2 which locate at the plasma membrane of cells adjacent to that of SWEETs located assume influx of sucrose from the apoplast in the caryopsis. It implies that SWEET and SUT together undertake efflux and influx of sucrose across the plasma membranes when the sugar traverses apoplastic space in developing caryopsis. These findings will hopefully elucidate the molecular mechanism of post-phloem sugar transport in rice caryopsis and facilitate the improvement of rice yield and quality by adjusting these gene’s expression in the future.

Biography:

Dr. Tatsuki Kunoh, Engineering Doctor (Ph.D. Engineering), now is a associate professor of Graduate School of Natural Science and Technology, Okayama University, Japan. He received his B. Sc. in biotechnology, M. Sc. and Ph.D. degrees in yeast cell biology at Osaka University. He accumulated his experiences in molecular biology and biochemistry in Albert Einstein College of Medicine, USA and other universities, Japan. Now, he is a member of the government-granted project, “Toward creating innovative applications to harness the novel functions of nano-scaled iron oxides of microbial origin” in CREST supported by JST. Currently his research focuses on the biomaterial science.           

Abstract:

So-called “green” synthesis of safe metal nanoparticles, especially gold nanoparticles (AuNPs), has increased in importance for medical and pharmaceutical applications; thus, a variety of ecofriendly, energy- and cost-saving techniques have been developed. Here we show that RNA prepared from Leptothrix (iron-oxidizing bacteria) cells can reduce Au(III), and spherical AuNPs eventually form when an aqueous solution of Au chloride (HAuCl₄ Soln) is added under ambient conditions. RNA and DNA of other organismal origins have the same ability. Of the nucleosides and nucleobases, only guanosine and guanine can form AuNPs. The DNA moiety, 2'-deoxyguanosine (dG) (used as a reference material), forms AuNPs when mixed with HAuCl4 Soln, but 8-hydroxy-2'-deoxyguanosine (8-OHdG) does not, indicating that AuNP-formation evidently depends on the reduction potential of the guanine moiety, not the sugar moiety. This finding is the first demonstration that spherical AuNPs of ca. 5 nm diameter can be obtained by simply adding guanine to HAuCl₄ Soln at ambient temperature; no other chemicals or physical treatments are needed.

Biography:

Dinh Thi My Huong completed her Barchelor from Da Nang University of Technology, recently she studies master program of Biochemistry at from Ming Chi Unviersity of Taiwan. She is the lecture of Da Nang collegue of Technology.

Abstract:

Water is the factor need to attend on the esterification to produce biodiesel by enzyme catalysts because of its complicated influences on the catalyst activity and sability of enzyme as well as the productivity of reaction. Some studies conclued that some lipases need to be maintained its proper structure by the certain amount of water. On the other hand, water is the by-product of the ester reaction, hence the high water content exists in the reaction media causes the back forward reaction, consequeces to reduce the yield. Therefore, this study drive to indentify the suitable amount of water for assisting activition of liquid enzyme catalyst, and investigate the using SAP to absorb the produced water. The effect of water content on the enzyme activity was assigned through discussed the efficiency of the esterifiaction with molar ratio of oleic acid to methanol 1:3 at the temperature of 250C, catalyst amount of 10wt% and varied water amount of 0 to 30 wt% (base on the fatty acid mass) for 1 hour and 3 hours. Another rections were carried out with the condition similar to above reactions and added 5% (w/woleic acid) of SAP amount to study the ability of maintaining the esterifiaction. Results illustrated that the conversion was increased obivously from 0% to 95 % with added water content increased from 0 wt% to 30wt%. Meanwhile the conversion rate was improved significant with using SAP, the yield reached to 95% with added water ammount of 10 wt% for only 1 hour.
 

Biography:

Gladys Hayashida has completed recently her PhD from Antofagasta University and her Master studies from Kyoto University. She is the director of the Associative Regional Project Explora of CONICYT, a Science Promotion Grant supported by the Goberment of Chile and implemented by the Antofagasta University. She has published scientific articles related to bioactive substances from marine bacteria and microalgal biotechnological applications, in reputed journals and has been her participating as researcher in several scientific studies.

Abstract:

Microalgae are an important source of unsaturated fatty acids, phospholipids, glycolipids, and carotenes, which are useful compounds for the food and pharmaceutical industries. The Atacama Desert of northern Chile is one of the driest deserts on Earth and, as such, it is a great natural laboratory in which to study new microorganisms adapted to extreme environments. A microalgal strain, referred to here as CH03, was isolated from a microbial mat in salt flat water in Salar de Atacama. Genetic analysis of the 18S ribosomal RNA gene showed that the strain had homology with other known sequences of the species Chlorella sorokiniana. Results revealed the adaptability of this microalga to freshwater medium under laboratory conditions, despite coming from an extremely high-salinity environment. The fatty acid profile of CH03(A) newly isolated in Bold's basal medium differed from that of CH03(B) cultured in vitro in modified F/2 medium and from another five strains of C. sorokiniana and three strains of Chlorella vulgaris in that it had a high stearic acid content and had no polyunsaturated fatty acids. The major biochemical components observed in this strain were proteins (64.3–73.6%) and lipids (26.6–32.6%). This study suggests that the strain CH03 could be a protein source and that this oleaginous microalga is easy to grow in vitro as a biological model for future studies.