Mahasin Ahmed Wadi
Princess Nourah Bint Abdulrahaman University, KSA
Title: Bioactivity of different bee honey samples
Biography
Biography: Mahasin Ahmed Wadi
Abstract
Background: The therapeutic value of honey was underlined in various literature. Honey was widely used in folk medicine throughout the world. Honey has been used to treat a number of clinical conditions: treatment of burns, wounds, peptic ulcers, gastritis, eye infection and sore throat. Antibacterial activity is attributed partially to the high osmolality of the sugar content of honey (Crane , 1975).
Additional antibacterial activity of honey was investigated by extraction and fractionation of honey by organic solvents. Honey; ethyl acetate extract revealed potent antibacterial activity (Wadi, 2016).
Objectives: The objective of the current prospective study is to determine the antimicrobial activity of fifteen different honey samples collected from different floral origin. To verify the nature of active fraction of bee honey.
Methods: Fifteen different raw bee honey samples were obtained from different countries, as well as commercially sold honey samples from the local market different brands, of different floral origin. In vitro antibacterial activity of bee honey, petroleum ether , diethyl ether and ethyl acetate extracts of honey samples were tested against five standard organisms ; Staphylococcus aureus : ATCC 29213, Staphylococcus Methicillin Resistant (MRSA), ATCC: 23591 Escherichia coli :ATCC 25922 Klebsiella pneumoniae : ATCC 700603 and Pseudomonas aeruginosa ATCC 27853 .
Results: All honey samples exerted inhibitory effects on both Gram –positive and negative organisms. The petroleum ether and diethyl ether fraction exerted no activity, while the aqueous residue exerted strong antibacterial activity. Ethyl acetate fraction showed strong antibacterial activity.
Conclusion: The findings of the current study confirmed that all honey samples exerted strong antibacterial activity. The chemical findings of this study indicates the presences of polar antibacterial agent (s) which is characterized by its extractability by ethyl acetate to the organic phase (Wadi 2016).