Baolin Zhang
Food and Drug Administration
Title: Glycosylation of therapeutic proteins: Functions and analytical innovation
Biography
Biography: Baolin Zhang
Abstract
Glycosylation is a critical quality attribute of therapeutic glycoproteins, including monoclonal antibodies (mAbs), which can directly affect product safety and efficacy. However, the complexity of protein glycosylation poses a daunting analytical challenge. The current methods for glycan analysis (e.g., mass spectrometry) consist of laborious enzymatic digestion protocols and analyses of the resulting free glycans and aglycosylated proteins. This presentation describes an innovative lectin-based microarray approach for high throughput glycan analysis of native proteins. Case studies of commercial therapeutic glycoproteins will be discussed to highlight the applications of the lectin microarray platforms for the characterization of biotechnology products.