Biotechnology

Biography

Biography: Pınar BALOGLU

Abstract

Wheat is an annual herbaceous plant whose breeding is done all over the world. Siyez wheat (Tiriticum monococcum) with 2n chromosomes is the first wheat variety which is known the oldest and taken culture. bZIP transcription factors have DNA binding motifs and form the leucine zipper dimerization. Most of WRKY type transcription factors control regulation of important functions for the development of plants. In this study, transcription factor genes were firstly cloned form einkorn wheat. For this, genes were amplified in PCR using gene specific primers with Pfu Taq Polymerase enzyme. PCR products were transferred to pENTR™/D-TOPO^® input vector. Plasmids containing genes were sent to sequencing. Finally, the sequences of the genes were aligned using CLC Genomics Workbench bioinformatics program. bZIP gene with 450 bp in length and WRKY gene with 672 bp in length were successfully cloned in a Gateway-compatible input vector. DNA sequence of genes were translated to protein sequence. A total of 150 amino acids in length for bZIP proteins and a total of 224 amino acids in length for WRKY proteins were determined. 3-D structure of proteins were modeled using Phyre2 program. The structural differences of these proteins in Siyez were identified. With this study, the sequence of bZIP and WRKY type transcription factors genes were firstly determined in ancestral wheat cultivar, Siyez and tried to obtain information about the function of these genes. Obtained results from this study may be used for development of abiotic stress resistance plants.