Day 2 :
Ming Chi University of Technology, Taiwan
Title: Effect of water and super adsorbent polymer (SAP) content on the efficiency of production biodiesel by liquid enzyme catalyst.
Time : 10:00-10:35
Dinh Thi My Huong completed her Barchelor from Da Nang University of Technology, recently she studies master program of Biochemistry at from Ming Chi Unviersity of Taiwan. She is the lecture of Da Nang collegue of Technology.
Water is the factor need to attend on the esterification to produce biodiesel by enzyme catalysts because of its complicated influences on the catalyst activity and sability of enzyme as well as the productivity of reaction. Some studies conclued that some lipases need to be maintained its proper structure by the certain amount of water. On the other hand, water is the by-product of the ester reaction, hence the high water content exists in the reaction media causes the back forward reaction, consequeces to reduce the yield. Therefore, this study drive to indentify the suitable amount of water for assisting activition of liquid enzyme catalyst, and investigate the using SAP to absorb the produced water. The effect of water content on the enzyme activity was assigned through discussed the efficiency of the esterifiaction with molar ratio of oleic acid to methanol 1:3 at the temperature of 250C, catalyst amount of 10wt% and varied water amount of 0 to 30 wt% (base on the fatty acid mass) for 1 hour and 3 hours. Another rections were carried out with the condition similar to above reactions and added 5% (w/woleic acid) of SAP amount to study the ability of maintaining the esterifiaction. Results illustrated that the conversion was increased obivously from 0% to 95 % with added water content increased from 0 wt% to 30wt%. Meanwhile the conversion rate was improved significant with using SAP, the yield reached to 95% with added water ammount of 10 wt% for only 1 hour.
- Environmental Biotechnology
Inha University, South Korea
Title: Characterization of microbial diverssity infl uenced by natural radon source in soil environment
Time : 10:35-11:10
Professor Kim and Kyu-yeon Lee have been studying on the neutralization ability of acid soil and the environmental impacts and decomposition mechanisms of micro pollutants such as medicines and micro-plastics in Soil Groundwater Laboratory of Inha University. Also, investigating on the characteristics of microbial diversity in natural radon soil environments verifying microbial differences at various radon concentration conditions.
Radon is an inert gas with no color and odor having a half-life of 4 days, which is a radioactive element produced by the decay of Uranium. Recently, public interest about indoor radon presence has been increased over decade. In general, high concentrations radon is generally known to be making deleterious effects on plants, animals and humans, which can cause cell viability disruption, cell morphological changes or hormonal disorders. On the contrary, lower concentration of radon may nevertheless improve crop growth while disabling pest activity. This study shows how much of lower level concentrations of radon in natural soils affect microbial community and their diversity with regard to basal soil physicochemical characteristics. Microorganisms exposed to low radioactivity, such as low-level radon, can have strong viability and high biodiversity. Soil physicochemical parameters such as pH, electrical conductivity, moisture content and soil particle size were measured according to Korean Standard Analytical Methods for Soils. Gas phase of radon concentration was measured for 1 hour (FRD-400, FT-Radon Lab., Korea) while the concentration of it has been varied in lower, equal and greater level compared to the natural source of radon origin in the field. In the meantime, colony enumeration, dehydrogenase activity, and identification of species were performed. In the lon run, there were relatively greater extent of diversity and population density being observed when microbes were exposed to relatively lower or equal level compared to the natural origin. In response, they revealed higher enzymatic activity under the given lower level radon exposure.
- Medical Biotechnology
Chulalongkorn University, Thailand
Title: Combination of bacterial cellulose wound dressing, silk sericin and polyhexamethylene biguanide for wound healing
Time : 11:30-12:05
Supamas Napavichayanun is a PhD student, faculty of Pharmaceutical Sciences, Chulalongkorn University, Thailand. She earned a B.Sc. from faculty of Phamaceutical sciences, Chulalongkorn University in 2010. Her research experience has ranged from protein including silk proteins and biomaterials. She also did clinical researches in the area of dermatology especially materials for wound healing application.
Wound dressing is an important factor for wound treatment. The good properties of wound dressing will lead to accelerate wound healing. Therefore, the objective of this study was to develop the bacterial cellulose wound dressing containing silk sericin and polyhexamethylene biguanide (PHMB) for wound treatment. Coconut is one of the value product of Thailand’s industrial harvests. Unfortunately, ripe coconut water is usually a waste product from coconut milk production that is discarded into the environment and results in pollution. Bacterial cellulose is produced by Acetobacter xylinum which is fermented in ripe coconut water. It has many advantages for wound healing: transparency, autolytic debridement, acceleration of re-epithelialization, and fewer daily wound dressing changes. Because of the many benefits of bacterial cellulose, it is used in many applications including cosmetics and medical devices such as wound treatment dressing material. Silk sericin is a protein from silk cocoons which can accelerate the proliferation of fibroblast cells and activate collagen synthesis for wound healing. PHMB is a broad-spectrum antimicrobial agent with high efficacy and low toxicity. Accordingly, the combination of bacterial cellulose wound dressing containing silk sericin and PHMB will have many benefits to the wound. The safety and efficacy of the dressings, in vitro and in vivo was investigated. The results showed the dressing has good physical, mechanical, and biological properties. The wound size of wounds treated with the dressing showed a significantly less than control. No inflammation or irritation was shown in rats. Moreover, in the clinical study, the dressing also showed many benefits for split–thickness skin graft wound treatment without any toxicity.
- Animal Biotechnology
Razi Vaccine and Serum Research Institute, Iran
Title: A novel marvelous evolutionary detection: lack of a large 2888 bp intron region within HA03 gene from Hyalomma anatolicum anatolicum, unlike its commercial recombinant anti-tick orthologue, Bm86, from Boophilus microplus
Time : 12:05-12:40
Khosrow Aghaiypour Kolyani has completed his PhD at the age of 30 years from Tehran University of Medical Sciences and postdoctoral studies from national Institute of Health, National Cancer Institute at frederick, USA. He is the head of Genomics and Genetic Engineering Department of Razi Vaccine and Serum Research Institute, which organizes the main human and animal vaccine research in Iran. He has published more than 30 papers in reputed journals and has been serving as an editorial board member of repute.
Hyalomma anatolicum anatolicum (H. a. anatolicum) as the most widespread tick species in Iran and other parts of the middle east is responsible for the hugely serious economic losses in livestock industry. This study was conducted to investigate genetic variability of the Bm86 orthologous gene, HA03, in five different Iranian H. a. anatolicum isolates including Kordan, Qom, Boinzahra, Lorestan and Bushehr. Likewise, a number of in silico analyses were performed in order to predict the possible impact of the amino acid substitutions on antigenicity of the protein. Comparative sequence analysis of the Bm86 orthologous gene sequence among five tick isolates allowed for identification of four non-synonymous single nucleotide polymorphisms (SNPs) including c.995A > C, c.1150G > C, c.1151A > C/T and c.1152G > T which would result in p.Asn 332 Thr, p.Glu 384 Leu and p.Glu 384 Ala substitutions. As much as antigenicity is concerned, based on our in silico studies, the amino acid position 384 was located in a putative antigenic peptide of the protein. Our subsequent physicochemical and structural analyses illustrated that two out of three amino acid substitutions including p.Glu 384 Leu and p.Glu 384 Ala considerably influenced the 3-dimensional structure and physicochemical properties of HA03 protein including hydrophobicity, amphiphilicity and net charge; thus, they might affect the antigen-antibody reaction and consequently immunogenicity of the antigen. In conclusion, it is a rational measure not only to replace Bm86 with HA03 in formulation of the recombinant anti-tick vaccine, but also to combine various antigens extracted from different isolates of the tick species.
Mahasin wadi PhD Medical Microbiology, is currently working at College of Nursing , Princess Nourah Bint Abdulrahaman University, Riyadh, Saudi Arabia. She published a numbers of papers in reputed journals and Participated in many Internationals and national conferences. She issued a patent research about the antimicrobial activity of Sudanese bee honey. She attended many workshops and seminars. Awarded many appreciation and thanks certificate in scientific activities. Member of many international associations : German Apitherapy Society, American Apitherapy Society, International Bee Research Association , European Society of Clinical Microbiology and Infectious Disease ESCMID . She served as reviewer of various journals. Her research interest area is the antibacterial and haling effects of Bee honey as natural product.
Background: The therapeutic value of honey was underlined in various literature. Honey was widely used in folk medicine throughout the world. Honey has been used to treat a number of clinical conditions: treatment of burns, wounds, peptic ulcers, gastritis, eye infection and sore throat. Antibacterial activity is attributed partially to the high osmolality of the sugar content of honey (Crane , 1975).
Additional antibacterial activity of honey was investigated by extraction and fractionation of honey by organic solvents. Honey; ethyl acetate extract revealed potent antibacterial activity (Wadi, 2016).
Objectives: The objective of the current prospective study is to determine the antimicrobial activity of fifteen different honey samples collected from different floral origin. To verify the nature of active fraction of bee honey.
Methods: Fifteen different raw bee honey samples were obtained from different countries, as well as commercially sold honey samples from the local market different brands, of different floral origin. In vitro antibacterial activity of bee honey, petroleum ether , diethyl ether and ethyl acetate extracts of honey samples were tested against five standard organisms ; Staphylococcus aureus : ATCC 29213, Staphylococcus Methicillin Resistant (MRSA), ATCC: 23591 Escherichia coli :ATCC 25922 Klebsiella pneumoniae : ATCC 700603 and Pseudomonas aeruginosa ATCC 27853 .
Results: All honey samples exerted inhibitory effects on both Gram –positive and negative organisms. The petroleum ether and diethyl ether fraction exerted no activity, while the aqueous residue exerted strong antibacterial activity. Ethyl acetate fraction showed strong antibacterial activity.
Conclusion: The findings of the current study confirmed that all honey samples exerted strong antibacterial activity. The chemical findings of this study indicates the presences of polar antibacterial agent (s) which is characterized by its extractability by ethyl acetate to the organic phase (Wadi 2016).
- Industrial and Microbial Biotechnology
Jiangnan University, China
Title: Effect of surfactants on the production and structure of Curdlan from Agrobacterium sp. ATCC 31749
Time : 14:15-14:50
Xiaobei Zhan completed his PhD from Kansans State University in 2004, and is a professor of fermentation engineering from Jiangnan University. He is the director of Industrial Microbiology and Biological Reaction Engineering Research Center, School of Biotechnology, Jiangnan University and the deputy Director of Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education. He is also the ASAE and IFT Institute of United States. His research focus is technology of high-viscosity fermentation, biosynthesis of microbial polysaccharide, preparation of functional oligosaccharide and design of bioreactor. He has published more than 30 papers in reputed journals.
The serious envelopment of curdlan on Agrobacterium sp. ATCC 31749 during fermentation is a major obstacle to increase curdlan production, and the compact macrostructure of curdlan produced from Agrobacterium sp. ATCC 31749 limits its application in preparing curdlan β-glucooligosaccharides. In this study, the effects of different surfactants on curdlan production by Agrobacterium sp. ATCC 31749 was investigated and several new perspectives concerned with the effect of Tween 80 promoting curdlan production and altering curdlan structure were presented. Maximum curdlan production (51.94 g/L) was achieved when 16 g/L Tween-80 was added at the beginning of the cell growth stage. The addition of Tween-80 at higher concentration inhibited cell growth. However, the addition of 16 g/L Tween-80 enhanced the production of curdlan with a looser ultrastructure, significantly weakened the envelopment of curdlan on Agrobacterium sp. ATCC 31749, altered the fine structure of cell membrane, and increased the cell membrane permeability. Compared with commercial curdlan, the curdlan with a looser ultrastructure exhibited higher substrate-binding affinity and maximum reaction rate when it was used as the substrate for preparing curdlan β-glucooligosaccharides. These findings demonstrate the mechanisms by which Tween-80 enhances curdlan production and provide a cheap and feasible approach to weaken the envelopment of water-insoluble polysaccharides on bacteria. Meanwhile, this paper provided an effective method to produce an ideal substrate so as to prepare oligosaccharides using enzymic degradation.
- Plant Biotechnology
Indonesian Industrial and Beverage Crops Research Institute (IIBCRI), Indonesia
Title: Complete Chloroplast Genome Sequences of cacao (Theobroma cacao L.) useful for Phylogenetic Analysis and DNA Barcoding
Time : 14:45-15:20
Nur Kholilatul Izzah has completed her PhD at the age of 37 years from Seoul National University (SNU) in 2014. She is researcher at Plant Breeding division, Indonesian Industrial and Beverage Crops Research Institute (IIBCRI), Indonesian Agency for Agricultural Research and Development (IAARD) under Ministry of Agriculture. In 2016, she conducted postdoctoral study at Functional Genomics Laboratory, Dept. of Plant Sciences, College of Agriculture and Life Sciences, Seoul National University for 7 months. She has published more than 8 papers in reputed journals and has been serving as an editorial team member of Journal of Industrial and Beverage Crops.
Cacao (Theobroma cacao L.) is known as main material for chocolate industry worldwide. Indonesia is recognized as the third largest cacao producer in the world with the total production in 2014 reached 709.331 tonnes. In order to understand cacao genomic, we conducted chloroplast genome sequencing generated by an Illumina Miseq platform. Chloroplast plays a crucial role in sustaining life on earth. The availability of chloroplast sequences could enhanced our understanding of chloroplast biology, conservation, diversity, and the genetic basis by which chloroplast transgenes can be engineered to enhance plant agronomic traits. The size of chloroplast genomes of cacao ranged from 160,619 bp to 160,649 bp. Cacao chloroplast sequences encoded 114 genes, consisted of 80 protein coding genes, 30 tRNA genes, and four rRNAs genes. Based on chloroplast sequences, we conducted phylogenetic analysis of 12 cacao genotypes that successfully separated bulk and fine types. The dendrogram resulted in this study proved the utility of chloroplast sequences for phylogenetic analysis. Some variations demonstrated through the number and structure of repetitive sequence in cacao chloroplast sequences. Identification of repetitive sequence by REPuter program exhibited that cacao possessed 18 repeats and three repeat structures (forward, palindrome, and reverse). In addition, we have developed three indel-based barcode markers which were designed based on the polymorphic regions of trnK-UUU - rps16, rps16 intron, and trnA-UGC - rrn23. The result obtained herein would give new insight regarding chloroplast genome structure in cacao, which would be useful to resolve phylogenetic relationships and development of DNA barcode markers.
Nanjing Agricultural University, China
Time : 15:20-15:55
Yibing Hu completed his Ph.D. in 2007, from Institute of Botany, Chinese Academy of Sciences. He is an associate professor of Nanjing Agricultural University with more than 20 papers in related journals .
Using β-glucoronidase (GUS) and Green Fluorescent Protein (GFP) represented expression, CRISPR-associated gene editing, cross-fertilization and determination of sugar related physiological parameters in gene mutant lines and wild type plants, we aimed to investigate the function of sugar transporter OsSWEETs and OsSUTs in rice caryopsis development during grain filling. Currently, we demonstrated that OsSWEET11 play an essential role in sucrose release from maternal tissue to the maternal–ﬁlial interface. It might also induce sucrose release from the ovular vascular trace and cross cells of developing caryopsis. In addition, OsSWEET15, a homolog of OsSWEET11 in rice SWEET family, also play an important part in grain filling besides its prominent role in pollen development of rice. By contrast, the sucrose-proton symporters OsSUT1/2 which locate at the plasma membrane of cells adjacent to that of SWEETs located assume influx of sucrose from the apoplast in the caryopsis. It implies that SWEET and SUT together undertake efflux and influx of sucrose across the plasma membranes when the sugar traverses apoplastic space in developing caryopsis. These ﬁndings will hopefully elucidate the molecular mechanism of post-phloem sugar transport in rice caryopsis and facilitate the improvement of rice yield and quality by adjusting these gene’s expression in the future.